Research Articles
BE4max vs. Sdd7: A Comprehensive Guide to Choosing the Right Cytosine Base Editor for Your Research
This article provides a detailed comparison of two premier cytosine base editors, BE4max and Sdd7, tailored for researchers and drug developers.
Targeting BCL11A for Sickle Cell Disease Therapy: From Mechanism to Clinical Applications
This comprehensive review details the pivotal role of the BCL11A transcription factor in silencing fetal hemoglobin (HbF) and its emergence as a prime therapeutic target for sickle cell disease (SCD).
Complete Guide to BCL11A Enhancer Editing: Protocol for Exa-cel (exagamglogene autotemcel) Development and Optimization
This comprehensive guide details the protocol for editing the BCL11A enhancer region to produce exagamglogene autotemcel (exa-cel), an autologous CRISPR-Cas9 edited cell therapy for sickle cell disease and beta-thalassemia.
Amplicon Sequencing for CRISPR Off-Target Analysis: A Comprehensive Guide for Therapeutic Developers
This article provides a complete framework for implementing amplicon sequencing to assess candidate off-target sites for CRISPR-based therapies.
Alt-R HDR Enhancer Protein: A Comprehensive Protocol Guide for Genome Editing Efficiency
This article provides a detailed, up-to-date protocol and analysis of the Alt-R HDR Enhancer Protein, a key reagent for enhancing Homology-Directed Repair (HDR) in CRISPR-Cas9 genome editing.
AiCErec: How AI is Revolutionizing Recombinase Engineering for Next-Gen Therapeutics
This article explores AiCErec, a cutting-edge AI-assisted platform for recombinase engineering, tailored for researchers and drug development professionals.
Agrobacterium-mediated Transformation of Embryogenic Cell Suspensions: A Complete Protocol for High-Efficiency Genetic Engineering
This comprehensive guide details the optimized protocol for Agrobacterium-mediated transformation of embryogenic cell suspensions (ECS), a cornerstone technique for advanced plant genetic engineering and molecular pharming.
Agrobacterium-Mediated CRISPR-Cas9 Delivery: A Comprehensive Guide for Genome Editing in Plants and Beyond
This article provides a detailed guide for researchers and biotechnologists on utilizing Agrobacterium tumefaciens as a vector for CRISPR-Cas9 genome editing.
Agrobacterium vs. Biolistic Delivery: Which Method Maximizes CRISPR Efficiency in Research & Drug Development?
This comprehensive analysis explores the critical choice between Agrobacterium-mediated transformation and biolistic delivery for CRISPR-Cas genome editing.
CRISPR Delivery via Agrobacterium: A Comprehensive Guide to Strain Selection for Plant Genome Engineering
This article provides a detailed guide for researchers and biotechnologists on selecting and optimizing Agrobacterium strains for efficient CRISPR-Cas delivery in plants.