Research Articles
Mastering Agrobacterium-Mediated Transformation: A Strategic Guide to Efficiency Across Plant Species and Genotypes for Biomedical Research
This article provides a comprehensive, up-to-date analysis of Agrobacterium-mediated transformation efficiency, a cornerstone technique for plant genetic engineering with critical applications in molecular pharming and drug development.
The Ti Plasmid's T-DNA Borders: From Plant Transformation Engine to Advanced Biomedical Tool
This comprehensive review explores the Agrobacterium tumefaciens Ti plasmid and its T-DNA border sequences, detailing their foundational biology and their pivotal evolution into sophisticated genetic engineering vectors.
Unlocking Genome Editing Potential: Strategies to Overcome Restrictive PAM Requirements for Higher Efficiency
This article provides a comprehensive guide for researchers and drug development professionals on addressing the critical bottleneck of low editing efficiency caused by the Protospacer Adjacent Motif (PAM) requirements of...
Designer vs. Natural: Unpacking the Next Generation of High-Fidelity Cas9 Variants for Precision Genome Editing
This article provides a comprehensive analysis for researchers and drug developers on the evolution of Cas9 enzyme specificity, contrasting naturally evolved variants with those engineered through computational and AI-driven protein...
Beyond Natural Limits: How AI-Designed Cas9 Variants Are Revolutionizing Genome Editing
This article provides a comprehensive analysis for researchers and drug development professionals on the emerging paradigm shift from natural Cas9 nucleases to AI-designed variants.
ABE8e vs ABE-NW1: A Comprehensive Comparison of A-to-G Base Editing Specificity and Precision
This article provides a detailed technical comparison of two prominent Adenine Base Editors (ABEs), ABE8e and ABE-NW1, focusing on their editing specificity, precision, and implications for research and therapeutic development.
Choosing the Right Viral Vector for Long-Term CRISPR Expression: An In-Depth Comparison of AAV vs. Lentiviral Systems
For researchers and drug developers engineering long-term gene editing therapies, the choice between Adeno-Associated Virus (AAV) and lentiviral vectors for sustained CRISPR-Cas expression is critical.
The 4.7kb Bottleneck: Understanding and Overcoming AAV CRISPR Cargo Size Limitations in Gene Therapy
This article provides a comprehensive guide for researchers and drug developers on the critical size constraint (~4.7 kb) for packaging CRISPR-Cas systems into Adeno-Associated Virus (AAV) vectors.
Liver-Specific AAV Promoters for Cas9 Tolerance Induction: A Comprehensive Guide for Gene Therapy Researchers
This article provides a detailed examination of adeno-associated virus (AAV)-delivered, liver-specific promoters for inducing immune tolerance to CRISPR-Cas9.
AAV Vectors for Base Editing: A Comprehensive Guide for Therapeutic Gene Editing Research
This article provides a detailed guide for researchers and drug developers on utilizing Adeno-Associated Virus (AAV) vectors to deliver base editors for precise gene correction.