Research Articles
Cas9 vs Cas12a for Multiplex Editing: A Strategic Guide for Therapeutic Development
This article provides a comprehensive comparison of CRISPR-Cas9 and Cas12a systems, with a dedicated focus on their application in multiplex genome editing for biomedical research and therapeutic development.
Indel Formation Rates in Gene Editing: A Comparative Analysis of CRISPR, TALEN, ZFN, and Prime Editing Platforms
This article provides a comprehensive comparison of insertion/deletion (indel) formation rates across major gene-editing platforms, including CRISPR-Cas9, TALENs, ZFNs, base editors, and prime editors.
A Comprehensive Guide to Validating Gene Function with CRISPR Knockout Studies: From Design to Clinical Translation
This article provides researchers, scientists, and drug development professionals with a current and exhaustive framework for validating gene function using CRISPR knockout (KO) technologies.
Beyond Cre-Lox: How Next-Gen PCE Systems Are Revolutionizing Chromosome Engineering
This article provides a comprehensive analysis for researchers and drug development professionals on the pivotal shift from the foundational Cre-Lox system to the novel Programmable Chromosome Engineering (PCE) platforms.
Prime Editing vs. Base Editing: A Precision Genome Engineering Showdown
This article provides a comprehensive comparison of two leading precision genome editing technologies: base editing and prime editing.
Viral vs. Non-Viral CRISPR Delivery: A Comprehensive Guide for Therapeutic Development
This article provides a definitive comparison of viral and non-viral methods for delivering the CRISPR/Cas9 system, tailored for researchers and drug development professionals.
Cas Protein Variants Efficiency Comparison: A Strategic Guide for Therapeutic Development
This article provides a comprehensive analysis of the editing efficiency and specificity of diverse CRISPR-Cas protein variants, tailored for researchers and drug development professionals.
A Comprehensive Guide to Validating CRISPR Edits: From Sanger to NGS Sequencing Methods
This article provides researchers, scientists, and drug development professionals with a definitive guide to validating CRISPR-Cas9 gene edits.
High-Fidelity Cas Variants: A Comprehensive Guide to Enhancing CRISPR Specificity for Research and Therapy
This article provides a detailed guide for researchers and drug development professionals on leveraging high-fidelity Cas variants to overcome the critical challenge of off-target effects in CRISPR genome editing.
Strategies to Evade Immunity: Engineering Safer CRISPR-Cas9 Therapies
The immunogenicity of the Cas9 nuclease presents a significant challenge for the clinical application of in vivo CRISPR-Cas9 therapies.