Research Articles
High-Fidelity Cas Variants: A Comprehensive Guide to Enhancing CRISPR Specificity for Research and Therapy
This article provides a detailed guide for researchers and drug development professionals on leveraging high-fidelity Cas variants to overcome the critical challenge of off-target effects in CRISPR genome editing.
Strategies to Evade Immunity: Engineering Safer CRISPR-Cas9 Therapies
The immunogenicity of the Cas9 nuclease presents a significant challenge for the clinical application of in vivo CRISPR-Cas9 therapies.
Overcoming Mosaicism in CRISPR-Edited Organisms: Strategies for Reliable Gene Editing in Research and Therapy
This article provides a comprehensive analysis of genetic mosaicism, a prevalent challenge that reduces the efficiency and reliability of CRISPR-based gene editing in both model organisms and therapeutic applications.
Precision Delivery: Advanced Strategies to Enhance CRISPR Accuracy in Target Cells
This article provides a comprehensive overview of the critical link between delivery methods and the accuracy of CRISPR-Cas9 gene editing in target cells, tailored for researchers and drug development professionals.
Navigating the Landscape of Gene Editing Off-Target Detection: From Foundational Concepts to Clinical Validation
As CRISPR-based gene editing rapidly advances toward clinical applications, the precise detection of off-target effects has become a critical pillar for ensuring therapeutic safety and efficacy.
Advanced pegRNA Design Strategies: Maximizing Prime Editing Efficiency for Therapeutic Applications
Prime editing represents a transformative advance in precision genome editing, yet its efficacy is critically dependent on the design of the prime editing guide RNA (pegRNA).
Boosting CRISPR Efficiency: A Scientist's Guide to Troubleshooting Low Editing Rates
This guide provides researchers, scientists, and drug development professionals with a comprehensive framework for diagnosing and resolving low CRISPR-Cas9 editing efficiency.
Boosting CRISPR HDR Efficiency: Advanced Strategies for Precision Genome Editing in Research and Therapy
Homology-Directed Repair (HDR) is the cornerstone of precise CRISPR-Cas9 genome editing, enabling targeted gene insertions, corrections, and the creation of sophisticated disease models.
Strategies to Minimize CRISPR Off-Target Effects: A 2025 Guide for Therapeutic Development
This article provides researchers, scientists, and drug development professionals with a comprehensive, up-to-date guide on addressing CRISPR off-target effects.
CRISPR-Cas9 Gene Therapy Protocols for Sickle Cell Anemia: From Mechanism to Clinical Application
This comprehensive review examines current CRISPR-Cas9 gene therapy protocols for sickle cell anemia, targeting researchers and drug development professionals.