Research Articles
ABE8e vs ABE-NW1: A Comprehensive Comparison of A-to-G Base Editing Specificity and Precision
This article provides a detailed technical comparison of two prominent Adenine Base Editors (ABEs), ABE8e and ABE-NW1, focusing on their editing specificity, precision, and implications for research and therapeutic development.
Choosing the Right Viral Vector for Long-Term CRISPR Expression: An In-Depth Comparison of AAV vs. Lentiviral Systems
For researchers and drug developers engineering long-term gene editing therapies, the choice between Adeno-Associated Virus (AAV) and lentiviral vectors for sustained CRISPR-Cas expression is critical.
The 4.7kb Bottleneck: Understanding and Overcoming AAV CRISPR Cargo Size Limitations in Gene Therapy
This article provides a comprehensive guide for researchers and drug developers on the critical size constraint (~4.7 kb) for packaging CRISPR-Cas systems into Adeno-Associated Virus (AAV) vectors.
Liver-Specific AAV Promoters for Cas9 Tolerance Induction: A Comprehensive Guide for Gene Therapy Researchers
This article provides a detailed examination of adeno-associated virus (AAV)-delivered, liver-specific promoters for inducing immune tolerance to CRISPR-Cas9.
AAV Vectors for Base Editing: A Comprehensive Guide for Therapeutic Gene Editing Research
This article provides a detailed guide for researchers and drug developers on utilizing Adeno-Associated Virus (AAV) vectors to deliver base editors for precise gene correction.
CRISPR in a Capsule: How AAV-Delivered crRNA Arrays Are Revolutionizing In Vivo Cancer Model Development
This article explores the cutting-edge methodology of using recombinant Adeno-Associated Virus (AAV) vectors to deliver CRISPR crRNA arrays for in vivo cancer modeling.
CRISPR vs. TALENs vs. ZFNs: A 2025 Specificity and Application Comparison for Researchers
This article provides a comprehensive, up-to-date comparison of the specificity of the three primary genome-editing platforms: CRISPR, TALENs, and ZFNs.
Beyond 80%: Advanced Strategies for Maximizing Prime Editing Efficiency in Biomedical Research
Achieving prime editing efficiencies beyond 80% represents a critical frontier for therapeutic applications.
Lentiviral Delivery of pegRNAs for Prime Editing: A Comprehensive Guide to Efficient Genome Engineering
This article provides a comprehensive overview of lentiviral vectors for delivering prime editing guide RNAs (pegRNAs), a critical component for achieving efficient and precise genome modifications.
CRISPR-Cas9 Mechanism of Action: A Foundational Guide for Research and Therapeutic Development
This article provides a comprehensive exploration of the CRISPR-Cas9 mechanism of action, tailored for researchers, scientists, and drug development professionals.